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20 November 2018, Volume 25 Issue 11
    

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  • The Expression of CC Chemokine Ligand 18 in Hepatocellular Carcinoma and the Evaluation of Its Clinical Value
    AN Yang1,ZHAO Wen-chao1,QIU Bao-an1,ZHU Lin-zhong2,XIA Nian-xin1
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1577-1580. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.001
    Abstract ( ) Download PDF (3075KB) ( ) Knowledge map Save
    Objective To detect the expression of CC chemokine ligand 18 (CCL18) in hepatocellular carcinoma (HCC) and explore its role in the prognosis of cancer. Methods The immunohistochemical staining was performed to detect the expression of CCL18 in 64 pair of HCC and pericarcinomatous tissue collected from 2011 to 2012. The survival of each patient was followed up. The χ2 test was performed to compare the differences in the expression of CCL18 and Kaplan Meier method to analyze the survival status. The multivariate Cox regression was used for prognostic analysis. Results Strong positive, moderately positive, weakly positive and negative cases were 36, 14, 9 and 5 in cancer tissues, respectively, while  2, 9, 6 and 47  in pericarcinomatous tissue. CCL18 was highly expressed in cancer tissues which was in clinically staged I and II, also with low degree of tumor differentiation, no distant metastasis and with lymph node invasion. Patients with high CCL18 expression had a shorter survival period than that with low expression, and the CCL18 expression, cancer differentiation, staging were all important factors affecting the overall survival of HCC. Conclusion CCL18 could  be related with  the occurrence, development and prognosis of HCC, or it could serve as  a new biomarker for judging the prognosis of HCC.
  • An Analysis of Serum Cytokines Levels in HCV Related Mixed Cryoglobulinemia
    QIN Xu-zhen 1, LIU Peng2, ZHU Li3, LI Chao4, LI Jian5, SU Wei1
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1581-1585. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.002
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    Objective To investigate the serum cytokines levels in patients with Hepatitis C virus (HCV) related Mixed cryoglobulinemia(MC). Methods Matched withage, gender, HCV antibody test and the qualitative results of cryoglobulin, 30 patients were enrolled in this study. They were classified as MC and HCV double positive group (MC+HCV+), MC and HCV double negative group (MC-HCV-), MC positive HCV negative group (MC-HCV+), and MC negative HCV positive group (MC-HCV+). Westergren sedimentation method and capillary electrophoresistyping techniques were adopted for thequalitative,quantitative and classificationanalysisof cryoglobulin. Meso Scale Discovery (MSD) highsensitivity electrochemical luminescence was used to mearsure the serum levels of IL-2, CXCL10, CXCL12, and TSLP. We analized the data usingIBM SPSS 22.0 software. ResultsThe IL-2, CXCL12 and TSLP concentrations in MC+ group were higher than that of MC- group (P<0.05), and the CXCL12 and TSLP concentrationsin HCV-MC+ subgroupwere higher than that of HCV-MC-subgroup (P<0.05). There were no statistical differences in the serum levels of cytokines between other subgroups. Conclusion CXCL12, TSLPconcentrationsareassociated with MC, butare not related to HCV infection. These findings provide a preliminary basis for further research on the pathogensis of HCV related MC.
  • An Analysis on the Correlations Between Total Testosterone Level and Glucose, Insulin and Sex Hormones Levels in Women with Polycystic Ovary Syndrome
    ZHAN Si-en, ZHAI Yan-hong, CAO Zheng
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1586-1588. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.003
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    Objectives To study the associations of total testosterone（TT）level and the serum glucose, insulin and sex hormones levels in women with polycystic ovary (PCOS). Methods One hundred and ninety-four untreated women with PCOS were enrolled for the study. The levels of serum TT, glucose, insulin and sex hormones [dehydroepiandrosterone sulfate (DHEAS), sex hormone binding globulin(SHBG), follicle stimulating hormone(FSH), luteinizing hormone( LH), estradiol(E2), progesterone(PO) and prolactin(PRL)] were measured , and the results were then analyzed. Results The BMI and DHEAS levels were significantly higher, while the age and PO levels were significantly lower in the higher TT Group compared with the lower TT group (P < 0.05). The BMI and DHEAS in the higher TT Group were 28.55（25.01-30.49）（kg/m2） and 218.00（155.00-327.00）μg/dL, respectively, and those in the lower TT Group were 27.06（23.00-29.30）kg/m2 and 198.00（142.00-254.00）μg/dL, respectively. The age and PO in the higher TT Group were 26.1±4.8 years and 0.39（0.24-0.64）ng/mL, respectively,  and those in the lower TT Group were 27.5±4.1years and 0.46（0.32-1.10）ng/mL, respectively. The TT level was positively correlated with the DHEAS level, but inversely related to age（P<0.05）. The values of r were 0.221 and ?0.213, respectively. Conclusion The level of TT is not related to the serum levels of glucose and insulin, but the patients with high level of TT tend to be younger and more prone to obesity, and have poor sex hormone level.
  • An Analysis of Homologous Genotype and Drug Resistance of Acinetobacter Baumannii in Hospital
    GUO Jing-jing1,WANG Hui-zhu1,HUA Ming-xi2,HE Ming3,SUN Ji-yun1,ZHANG Shu-feng1,WANG Ya-jie1
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1589-1594. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.004
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    Objective To investigate the molecular epidemiology characteristic and analyze gene homologyof Acinetobacter baumannii isolated in each ward of our hospital, so to provide the knowledge evidences for nosocomial infectioncontrol, and the application of themultilocus sequence typing(MLST) technology.Methods 50 A．baumanni stains were collected from our hospital, and the genotype was identified by multilocus sequence typing(MLST)technique．gltA，gyrB，gdhB，recA，cpn60，gpi and rpoD housekeeping genes were detected by PCR (Polymerase Chain Reaction) amplification．PCR results were then sequenced andcompared with PubMLST databases．Evolutionary diagram was generated with Phyloviz 2.0 software．Results Tengenotypes were identified among 50 A．baumannii stains： ST - 1696 (19 strains), ST - 1144 (14 strains), ST - 191 (4 strains), ST-229 (4 strains) and ST - 540, ST - 1680, 208, ST - 1679, ST -1145, ST -1421 (1strains), respectively,and three new genes (3 strains). Among them, ST - 1696, and ST -1144, ST - 191, ST - 540, ST - 1680, ST - 208, ST - 1679, ST - 1145, ST - 1421 genotype have the homologous relevance.Drug sensitivity test results showed that most of 47 strains of acinetobacterbaumannii strains had multiple drug resistance of clinical commonly used antibiotics, and the antibiotic resistant rate were over 75%. Conclusion Acinetobacter baumannii isolated in our hospital have the homologous correlation, and MLSTcould be used in genotyping of clinical Acinetobacter baumannii.Multiple drug resistance of acinetobacterbaumanniishould be real-time detected and prevented.
  • A Study on the Correlation of ApoE Gene Polymorphism with the Age and Lipid Levels of Cerebral Infarction
    LIU Shu-jing, CHANG Xin-kui, CHEN Ke-lin, ZHANG Li-min, SI Xue-zhong, ZHANG Guo-jun
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1595-1598. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.005
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    Objective To investigate the relationship of ApoE gene polymorphism with the age and lipid levels in cerebral infarction patients. Methods A total of 221 patients with cerebral infarction were enrolled in the study. ApoE genotypes were detected by PCR fluorescent probe method. Patients were divide into group E2(E2E2 and E2E3), E3(E3E3) and E4(E4E4,E3E4) according to the ApoE genotypes, then test for TG, CHO, HDL, LDL, ApoA1 and ApoB in their serum. Qualitative analysis was performed by χ2 test, and quantitative data were checked by t test. Results 221 patients in Beijing Tiantan Hospital from Jan, 2017 to Dce, 2017 were studied. The frequencies of each genotype were: E2E2 genotype of 1.3%, E2E3 genotype of 8.1%, E3E3 genotype of 76.0%, E3E4 genotype of 14.0%, E4E4 genotype of 0, and E2E4 genotype of 0.5%; The percentage of E4 low age group(22.6%) was higher than the percentage of E3 low age group, P=0.037. The levels of CHO (3.54 ± 1.40 mmol/L) and LDL (2.13 ± 0.81 mmol/L) in the E2 group were lower than those in the E3 group (3.77 ± 0.93 mmol/L and 2.20 ± 0.78 mmol/L) , respectively. The P values were 0.007 and 0.020, respectively. There was no significant difference in blood lipid level between E3 group and E4 group in low age group. The TG, CHO, LDL and ApoB in E4 high age group were 1.74 ± 0.57 mmol/L, 4.25 ± 1.23 mmol/L, 2.61 ± 1.04 mmol/L, and 0.89 ± 0.30mmol/L, higher than those in E3 group (1.35±0.53 mmol/L, 3.80±0.90 mmol/L, 2.23±0.76 mmol/L, 0.79±0.24 mmol/L). P values between the two groups were 0.033, 0.009, 0.008, and 0.034. Conclusion ApoE gene polymorphism may affect the occurrence of cerebral infarction. Furthermore, the E2 gene has certain effect to reduce the blood lipid; E4 gene may be closely correlated with young adults with cerebral infarction in the low-age population, and may be involved in the occurrence of cerebral infarction by elevating blood lipids levels in patients of high-age.
  • The Diagnostic Value of Protein Electrophoresis Combined with Immunoglobulin Detection in  Kidney Disease
    TIAN Juan，LI Li
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1599-1602. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.006
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    Objective  To explore the diagnostic value of serum protein electrophoresis, serum immunoglobulin and urine immunoglobulin detection in kidney disease. Methods 98 patients and 102 healthy person were recruited in the study and the serum protein electrophoresis test was conducted, also the serum and urine immunoglobulin G (IgG), immunoglobulin A (IgA), immunoglobulin M (IgM) were measured . Results In the renal disease group, the densities of band α1, α2 and β were significantly higher while band γ were significantly lower, and the IgG band of serum immunoglobulin were higher than the control group（P<0.05）. The serum IgG and urine microprotein were significantly lower than the control group（P<0.01）.  There was  a negative correlation between the band A of serum protein electrophoresis and the IgG level of immunoglobulin detection. Conclusions The protein electrophoresis combined with immunoglobulin detection have a significant diagnostic value in  the renal disease.
  • The Detection and Analysis of Blood Coagulation Parameters and Platelet Parameters in Patients with Liver Cirrhosis or Chronic Hepatitis B
    SUN Yan-hong,ZHONG Wei,ZHU Qiu-jie
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1603-1606. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.007
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    Objective To investigate the relationships between blood coagulation indexes and platelet parameters of various types of liver diseases. Methods 83 inpatients having  liver disease collected from January 2016 to December 2017 in our hospital were divided into the chronic hepatitis B group (32 cases) and the liver cirrhosis group (51 cases), while the  control group(85 cases of non-liver disease patients) were collected at the same period. The results of prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen (FIB), along with  platelet count (PLT), platelet distribution width (PDW), mean platelet volume (MPV) and platelet hematocrit level (PCT) were retrospectively analyzed. Results(1)Compared with the control group, PT and APTT in patients with chronic hepatitis B and cirrhosis were increased ,and FIB were decreased with  significant differences(P<0.05); Compared with the chronic hepatitis B group, APTT, PT and FIB in the liver cirrhosis groups were significantly different (P<0.05). (2)Compared with the control group, the PLT and PCT levels in the liver cirrhosis and chronic hepatitis B group decreased (P<0.05), and MPV, and PDW  increased significantly. The PLT and PCT levels in liver cirrhosis were  lower than those of chronic hepatitis B，while  MPV was higher than those of chronic hepatitis B.  Conclusion Platelet parameters and coagulation indicators will be varied in different types of liver diseases and can be useful in clinical diagnosis, prognosis and treatment of liver diseases.
  • A Study on the Changes of T/B/NK Lymphocyte Subsets in Peripheral Blood of Patients with Colorectal Cancer Before and after Chemotherapy
    LI Shi-long1,ZHANG Bao2,SONG Yang1,ZHANGHai-guang1,QIN Li3,WU Dan2,MIAO Ya-jing1
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1607-1610. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.008
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    Objective To explore the difference of the absolute numbers of T/B/NK lymphocyte subsets between preoperative colorectal cancer patients and healthy controls, and the effect of oxaliplatin and capecitabine chemotherapy on T/B/NK lymphocyte subsets in peripheral blood of patients with colorectal cancer after the operation. Methods The flow cytometry was used to examine 86 cases of colorectal cancer hospitalized in Tangshan City people's Hospital from May 2017 to January 2018. The peripheral blood T/B/NK lymphocyte subsets were detected before and after the chemotherapy. The T/B/NK lymphocyte subsets in the peripheral blood of the healthy control group were detected at the same time, and the SPSS24.0 statistics software was applied for the statistical analysis of the collected data. Results1 The absolute numbers of CD3+, CD3+CD4+, CD3+CD8+, CD3-CD19+ and CD16+CD56+ cells in the peripheral blood of colorectal cancer patients and the healthy control group were  significantly different (P<0.001). The levels of CD3+CD4+/CD3+CD8+, CD3-CD19+ and CD16+CD56+ in colorectal cancer patients were  not related with  the age, sex, and tumor location,  but  correlated with TNM staging. 2 The absolute numbers of CD3+, CD3+CD4+, CD3-CD19+and CD16+CD56 cells in peripheral blood decreased significantly in patients with colorectal cancer after the oxaliplatin and capecitabine, (P<0.001), and the absolute number of CD3+CD8+ cells was significantly higher than that of  pre-chemotherapy (t=4.167,P<0.001).Conclusion The levels of T/B/NK lymphocyte subsets in peripheral blood are   closely related with  colorectal cancer; the absolute numbers of T/B/NK lymphocyte subsets in peripheral blood of colorectal cancer patients decrease  significantly after the oxaliplatin and capecitabine, and the function of cell immunity, humoral immunity and natural killer cells decrease significantly.
  • Study on the Relationship Between Serum sVCAM-1、TNF- α、HDL-C and NF-κB in Patients with Coronary Heart Disease
    CUI Xiang
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1611-1614. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.009
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    Objective To investigate the correlation between serum sVCAM-1, TNF- α, HDL-C and NF-κB in patients with coronary heart disease. Methods The clinical data of 130 patients with coronary heart disease from September 2016 to September 2017 in our hospital were studied prospectively. The basic data, biochemical indexes, Spearman correlation analysis and Logistic multivariate regression analysis were carried out in all patients and 50 healthy persons who were examined in the same period as control group. Results The history of smoking, hypertension, diabetes, TG、LDL-C、sVCAM-1、TNF-α、NF-κB in the CHD group were significantly higher than those in the control group, and HDL-C was significantly lower than that in control group (P<0.05). The levels of serum sVCAM-1, TNF-α, NF-κB in AMI group were significantly higher than those in SAP group and UAP group (P<0.05). The levels of serum sVCAM-1, TNF-α, NF-κB in UAP group were significantly higher than those in SAP group (P<0.05), but there was no significant difference in HDL-C between the two groups (P>0.05). After pearson correlation analysis, there was a positive correlation between sVCAM-1, TNF-α and NF-κB in patients with coronary heart disease. HDL-C was negatively correlated with NF-κB in patients with coronary heart disease. Multivariate regression analysis showed that sVCAM-1, TNF-α and NF-κB were independent risk factors of coronary heart disease, and HDL-C was protective factor. Conclusion sVCAM-1, TNF-α, HDL-C and NF-κB are closely related to the occurrence and development of coronary heart disease, among which NF-κB is the key transcription factor to trigger inflammation. It may regulate the expression of sVCAM-1, TNF-α, HDL-C and affect the development of coronary heart disease.
  • Effects of Telbivudine or Entecavir Combined with Adefovir Dipivoxil on Liver Function, Hepatic Fibrosis Indexes, HBV-DNA Level and Adverse Reactions in Patients with Chronic Hepatitis B
    ZHANG Li-li, MA Huan, YANG Jian-bo, LUO Yu-jun
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1615-1618. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.010
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    Objective To observe the effects of telbivudine or entecavir combined with adefovir dipivoxil on liver function, hepatic fibrosis indexes, HBV-DNA level and adverse reactions in patients with chronic hepatitis B (CHB). Methods 304 cases of CHB patients were selected and randomly divided into two groups (each n=152). The control group was given 600 mg/d telbivudine and 10 mg/d adefovir dipivoxil, while the observation group was given 0.5 mg/d entecavir and 10 mg/d adefovir dipivoxil. The two groups were treated for 48 weeks. The liver function, hepatic fibrosis indexes, HBV-DNA level and adverse reactions were analysed in the two groups after 48 weeks of treatment. Results The levels of alanine transaminase (ALT) and aspartate aminotransferase (AST), alkaline phosphatase (ALP) and total bilirubin (TBIL) and the score of Child-Pugh decreased significantly compared with those before treatment (P<0.05), and the change degree in the observation group after treatment was higher than that in the control group (P<0.05). The levels of total protein (TP), albumin (ALB), and prothrombin activity (PTA) in the two groups were significantly higher than those before treatment (P<0.05), and the change degree in the observation group after treatment was higher than that in the control group (P<0.05). The levels of hyaluronidase (HA), laminin (LN), procollagen III(PCIII), collagen type IV (IV-C) and prolidase (PLD) in the two groups were significantly lower than those before treatment (P<0.05), and the change degree in the observation group after treatment was higher than that in the control group (P<0.05). The DNA negative rate of HBV and the seroconversion rate of HBeAg in the two groups were significantly higher than those before treatment (P<0.05), and the change degree in the observation group after treatment was higher than that in the control group (P<0.05). Conclusions Entecavir and adefovir dipivoxil can have better efficacy than telbivudine and adefovir dipivoxil in the treatment of CHB, the former one can have faster recovery of liver function, higher DNA negative rate of HBV and seroconversion rate of HBeAg, and have better safety and fewer adverse reactions, therefore the former one is worthy of clinical promotion.
  • The Expression of miRNA-146a in HPV-B19 infected patients after renal transplantation
    TANG Qian,ZHU Lei,DENG Xian-ping,LIU Xin-xin
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1619-1522. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.011
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    Objective To investigate the states of human parovirus(HPV)-B19 infection of recipients after the renal transplantation, and to study the functional mechanism of miRNA 146 in HPV-B19 infection of recipients after the renal transplantation. Methods We obtained patient serum samples at 3, 7, 10,15 and 25 days after the transplantation in 34 renal transplant recipients as the study group, while 34 samples before the transplantation were used as the control group for non-renal transplant and also 20 cases of healthy controls group. The HPV-B19 IgM ELISA assay was conducted in  blood serum and we thencompared the results among three groups; PCR was used to detect the levels of microRNA-146a for further comparison. Results The relative expression of transplantation recipients group(11.16±4.41) was significantly higher than pre  transplantation control group (8.39±2.09) and then healthy control group(7.74±2.96), P<0.05,; The relative expression of miRNA-146a in active HPV-B19 infection group(Relative Expression, RE )( 2.48×102±265.49) was significantly higher than  that of the taintless HPV-B19 group (4.77±3.23), P=0.013.; The correlation coefficient between  the expression of miRNA-146a and HPV-B19 infection group was R=0.076, P<0.05,.Conclusion There is a higher chance of HPV-B19 infection after the renal transplantation. miRNA-146a can be used as a potential diagnostic marker in HPV-B19 infection of recipients after the renal transplantation.
  • The Detection of Apolipoprotein CIII and Its Correlation Analysis with Blood Lipid in Patients with Coronary Heart Disease
    HUANG Zi-chu
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1623-1626. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.012
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    Objective To investigate the expression of apolipoprotein C III (Apo CIII) in patients with coronary heart disease and its relationship with serum lipids. Methods From January 2015 to January 2017, 120 patients with coronary heart disease admitted to the Department of Cardiology were enrolled as the observation group. Eighty patients with non-CHD who were hospitalized during the same period were selected as the control group. In the observation group, 3 mL of fasting venous blood was collected in the morning of the following day after the admission, while 3 mL of venous blood was taken from the abdomen in the control group, and plasma Apo CIII level was determined by immunoturbidimetry using the biochemical analyzer after the serum was separated. Using the American BECKMAN DxC800 biochemical analyzer, the levels of TC, TG, LDL-C, HDL-C, Apo AI and ApoB in two groups were measured. The patients in the observation group were treated with statins. After the treatment, the levels of TC, TG, Plasma Apo CIII were measured; SPSS Pearson correlation analysis software was used the analysis of apolipoprotein C III and coronary heart disease blood lipid levels. Results The levels of TC, LDL-C, ApoB and Apo CIII in the observation group were significantly higher than those in the control group (P <0.05), while the levels of TG and HDL-C in the observation group and the control group were not statistically different. The level of Apo AI and Apo AI in the observation group were lower than those in the control group (P <0.05). The levels of TG and HDL-C in the observation group before treatment and after treatment were not statistically different (P> 0.05) (P <0.05). The level of Apo AI in the observation group after treatment was higher than that of before treatment (P <0.05). SPSS Pearson correlation analysis showed that there were no correlations between levels of Apo CIII and TG and HDL-C (P> 0.05). There was a positive correlation between TC, LDL-C and ApoB levels in traditional blood lipids of patients with coronary heart disease (P <0.05). In patients with coronary heart disease, the Apolipoprotein CIII levels of traditional blood lipids in ApoB was negatively correlated (P <0.05). Conclusion The Apo CIII level can be detected accurately by immunoturbidimetry, and it has certain correlations with coronary heart disease and traditional blood lipid level. It is worth to promote the clinical application of Apo CIII and improving the clinical prognosis.
  • The Effect of NB-UVB on Psoriatic Peripheral Blood Th17 Cells and Serum IL-17, IL-22, IL-23 Expressions
    KUANG Wei-wei1,WEI Zhi-ping2,REN Hong1
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1627-1629. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.013
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    Objective To evaluate the clinical effect of narrow spectrum UVB (NB-UVB) in the treatment of psoriasis vulgaris and analyze the relevance?of Th17 cells and related cytokines before and after treatment with psoriasis area and severity (psoriasis area and severity index, PASI). Methods 40 cases of patients with psoriasis vulgaris treated with NB-UVB irradiation were selected as the treatment group and the efficacy was evaluated by PASI score. Another randomly selected 30 healthy persons were enrolled as the control group. The percentage of peripheral blood Th17 cells and the content of IL-17, IL-22 and IL-23 in the serum of the two groups were detected before and after the treatment, and the correlation between the levels of the PASI score before and after treatment were analyzed. Results The comparison results of Th17 cells before and after treatment: the percentage of Th17 cells pre-treatment was significantly higher than that of the healthy control group (P<0.05). After 12 weeks of phototherapy, the percentage of Th17 cells in peripheral blood decreased significantly compared with that of before phototherapy (P< 0.05). In addition, the levels of Thl7, IL-17, IL-22 and IL-23 were positively correlated with PASI scores before and after NB-UVB treatment (P<0.05). Conclusion NB-UVB irradiation can significantly decrease the levels of peripheral blood Th17 cells, along with levels of IL-17, IL-22 and IL-23, which may be one of the working mechanisms of NB-UVB in the treatment of psoriasis. NB-UVB has a positive curative effect and high safety in the treatment of psoriasis vulgaris.
  • Expression of RDWs-CRP in Patients with Coronary Heart Disease and its Correlation with Coronary Gensini Score
    WANG Peng-fei1,QIAO Hai-xia2,WANG Rui-juan1,DING Zhen-jiang1,HOU Rui-tian1
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1630-1633. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.014
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    Objective To investigate the expression of MPV,RDW and hs-CRP in patients with coronary heart disease and their relationship with coronary Gensini score.Methods 110 CHD patients treated in our hospital were enrolled in this study.110 cases of healthy people were selected in the same period as a control group.The analysis of single factor analysis and Logistic regression model,related risk factors of the two groups were observed.The mean platelet volume(MPV),erythrocyte distribution width(RDW)and hypersensitive C-reactive protein(hs-CRP)level under different Gensini integration were observed.Pearson correlation analysis was conducted for these factors.Results The smoking,hypertension,diabetes mellitus and MPV,RDW,hs-CRP and Gensini scores in the case group were significantly higher than those in the control group(P<0.05).Multivariate non-conditional Logistic regression analysis showed that smoking,hypertension,diabetes mellitus,MPV,RDW,and hs-CRP.All of them were independent risk factors of coronary heart disease.The indexes of MPV,RDW and hs-CRP in different Gensini score groups were different or not the same, and the difference was statistically significant(P<0.05).Pearson correlation test showed that there was a positive correlation between MPV,RDW,and hs-CRP. Conclusion MPV,RDW and hs-CRP are independent risk factors of coronary heart disease,and have a positive correlation with Gensini score,which can be used to evaluate the degree of occlusion of coronary artery.
  • The Clinical Values of Long Noncoding RNA SOX2OT, PVT1 and GHET1 in AFP Negative Hepatocellular Carcinoma
    LIU Xian-feng 1, XU Jie 1, ZHANG Xuan 2, WANG Hu-ming 2
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1634-1638. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.015
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    Objective To explore the clinical values of long noncoding RNAs (lncRNAs) SOX2OT, PVT1 and GHET1 in AFP negative liver cancer (AFP-NHCC). Methods 100 cases of AFP-NHCC diagnosed by puncture or pathological section were collected from July 2014 to December 2017. In the same period, 100 patients with benign liver diseases and 110 healthy persons in our hospital were collected. The expression differences between groups (such as SOX2OT, PVT1 and GHET1) were evaluated by Student’s t-test. The receiver operating characteristic (ROC) curve analysis was performed to estimate the diagnostic values of SOX2OT, PVT1 and GHET1 in AFP-NHCC. Multinomial logistic regression analysis was used to calculate the odds ratio (OR) and 95% confidence interval (CI). Results The expression levels of lncRNA SOX2OT, PVT1 and GHET1 in AFP-NHCC patients were higher than those patients with benign liver diseases and the healthy controls, (P<0.05). The expression levels of SOX2OT, PVT1 and GHET1 were linearly correlated with lymph node metastasis (SOX2OT：R2=0.321，P=0.002；PVT1：R2=0.305，P=0.006；GHET1：R2=0.286，P=0.011) and TNM classification (SOX2OT：R2=0.313，P=0.001；PVT1：R2=0.291，P=0.007；GHET1：R2=0.289，P=0.017) in AFP-NHCC. ROC curve analysis showed that SOX2OT, PVT1 and GHET1 was of high value in differentiating AFP-NHCC from patients with benign liver diseases and healthy controls, and the sensitivity/specificity were 67.7%/44.0%, 80.5%/61.0% and 79.2%/89.0%, respectively. After combining these three lncRNAs, the specificity was increased significantly, reaching 90.4%. Logistic regression analysis showed that the level of high SOX2OT, PVT1 and GHET1 was an independent risk factor for the onset of AFP-NHCC after the correction of factors such as age, sex, smoking and drinking. Conclusion The diagnostic value of plasma SOX2OT, PVT1 and GHET1 in AFP-NHCC is of great clinical significance in evaluating the distant metastasis of AFP-NHCC.
  • The Significance of Combined Detection of Serum FRA, Adropin and HE4 in the Diagnosis of Endometrial Cancer
    YANG Jing 1, ZHANG Hong 2
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1639-1643. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.016
    Abstract ( ) Download PDF (3667KB) ( ) Knowledge map Save
    Objective To investigate the significance of combined detection of serum folate receptor alpha (FRA), adropin protein and human serum epididymis 4 (HE4) in the diagnosis of endometrial carcinoma (EC). Methods 93 patients with EC (EC group), 96 patients with benign uterine diseases (benign uterine disease group) and 100 healthy women (control group) were selected as the subjects. Enzyme linked immunosorbent assay (ELISA) was used to detect the levels of serum FRA, adropin and HE4, and the correlationsamong the three indexes were analyzed. The subjects' work characteristic curve (ROC) and area under the curve (AUC)were used to evaluate the diagnosis value of EC. Results The serum FRA in EC group was 1614±191 pg/mL, adropin 1.94±0.75 ng/mL, and HE4 106.57 (22.39~1411.12)pmol/L, and those in the group of benign uterine diseases were 1142±156pg/mL, 9.07±2.57 ng/mLand 31.02 (13.05~163.02)pmol/L, while the control group were (448±153)pg/mL, (14.52±17.60) ng/ml, and5.61 (11.37~48.29)pmol/L.The FRA and HE4 of the EC group and the benign uterine disease group were significantly higher than those of the control group, and the adropin was significantly lower than that of the control group. The differences between the EC group and the benign uterine disease group were also statistically significant (P < 0.05), also the FRA and HE4 in the benign uterine disease group were significantly higher than those in the control group, while adropin was significantly lower than that of the control group (P < 0.05). In group EC, the serum FRA and HE4 of patients with clinical stage beyond stage II and deep myometrium infiltration were significantly higher than those of patients with stage I and superficial musculus infiltration, and adropin was significantly lower than those of stage I and superficial musculus infiltration patients(P< 0.05). There were no significant differences in serum FRA, adropin and HE4 levels across groups with different age, degrees of non-tumor differentiation, menopause and pathological type (P > 0.05). The serum levels of HE4 and FRA were positively correlated with the FRA (r=0.604, P＜0.001）, and HE4 and FRA were negatively correlated with adropin (r= 0.379, P=0.026; r= 0.442, P=0.002). The AUC and sensitivity of combined detection of FRA, HE4 and adropin in diagnosing EC were 0.951 (95%CI:0.893~0.980) and 91.96%. Conclusion The levels of serum adropin, FRA and HE4 in patients with EC are increased. These three indexes can be used as serological markers for EC, and the combined detection of markers is helpful for the diagnosis of endometrial carcinoma.
  • The Clinical Analysis of Biochemical Indicators and Tumor Markers of Human with Different Age and Gender
    GUO Fei1, GAO Yan-ling2, MEN Sha-sha1,-ZHANG Li-na1, GAO Yan-hong1
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1644-1649. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.017
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    Objective To explore the characteristics of biochemical indicators and tumor markers in different genders and ages. Methods The Roche COBAS 8000 automatic biochemical analyzer and immunoanalyzer were used to detect the biochemical indicators and tumor markers of 804 physical examination personnels and analyze the datas. Result  ALT、AST、TBA、GGT、CR、UA、CK、CA125、CEA of male were higher than those of female. HDL andCA199 of female were higher than those of male. The abnormal results were mainly concentrated in HDL (38.18%), LDL (29.48%), TG (28.36%), GLU (12.60%) and GGT (12.60%). The abnormal rates of TBA, GGT, GLU, BUN, CR, UA, CK, TG and CEA of male were all significantly higher than those of female, and the abnormality rates of HDL, CA125 and CA199 of female were significantly higher than those of male. The men and women were divided into three groups according to age. In comparison between the ages of 39-49 and 50-55, male had significant differences in GLU, and female had the significant differences in ALT, AST, TBA, ALP, GGT, BUN, UA, CH, TG, LDL, CK, LDH, AFP and CA125. In comparison between the ages of 50-55 and ≥ 56, the significant differences indicators of male were ALT, GGT, GLU, BUN, CH, TG, HDL, LDL, LDH, CEA and CA199 , and the significant differences of female were AST, BUN, CK, LDH and CEA. With the increase of age, the level of male ALT, GGT, CH, AFP decreased gradually, while the levels of GLU, BUN, LDH, CEA and CA153 showed a gradual upward trend. The level of female CA125 showed a gradual decline, while the indexes of ALT, AST, TBA, ALP, GGT, GLU, BUN, CR, UA, CH, TG, CK and LDH showed a gradual upward trend. Conclusion  The abnormality of glucose, blood lipid and liver function is the most common among the physical examination personnel, and it is more important for men to pay more attention to their glucose, kidney function and abnormal blood lipid metabolism. After age 56, men begin to show significant changes in their biochemical indicators and tumor markers, the significant differences of women  happen at about 50 years old, and the significant differences indicators in the number are more than male.
  • The Comparison of Clinical Efficacy, Fusion Gene,Serum Markers and Prognosis of Crizotinib and Non-crizotinib treatment for EML4-ALK Positive  Patients with Non Small Cell Lung Cancer
    WU Li
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1650-1653. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.018
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    Objective　To compare the clinical efficacy, fusion gene,serum markers and prognosis of crizotinib and non-crizotinib treatment for EML4-ALK positive patients with non-small cell lung cancer. Methods 60 cases of positive EML4-ALK NSCLC patients were selected as the research subjects.  These subjects were then divided into the study group and the control group with 30 cases each.Patients in the control group were given non-crizotinib treatment and patients in the study group were given the crizotinib treatment, The two groups were then compared with the  clinical efficacy and other factors . Results　 The clinical efficacy in the study group were significantly higher than the control group(P<0.05); The expression of EML4-ALK in the study group was significantly lower than the control group(P<0.05); The serum indexes were all lower in the study group than in the control group after the treatment（P＜0.05）; The prognosis in the study group were significantly higher than the control group(P<0.05) ; There was a significant negative correlation between the survival rate and the EML4-ALK expression level （r ＝–0.336，P＜0.05），and there was a significant positive correlation among  serum CEA, CYFRA21-1 and EML4-ALK expressions （r＝0.253, 0.438; P＜0.05）.Conclusion　 Crizotinib can decrease the level  of EML4-ALK in treating NSCLC patients and increase the prognosis. It is worthy of clinical promotion.
  • Clinical Significance of Serum SF and LDH and Morphological Features of Bone Marrow Cells in the Differential Diagnosis of Myelodysplastic Syndrome and Megaloblastic Anemia
    CHEN Li-juan, WU Ya-bing, HAN Shao-ling
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1654-1657. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.019
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    Objective  To explore the clinical significance of serum ferritin (SF), lactate dehydrogenase (LDH) and morphological features of bone marrow cells in the differential diagnosis of myelodysplastic syndrome (MDS) and megaloblastic anemia (MA). Methods 40 patients with MDS (MDS group) and 40 patients with MA (MA group) treated in the hospital from January 2013 to December 2016 were enrolled in this study. The clinical symptoms, results of peripheral blood smear examination and bone marrow cell morphology were compared between these two groups. The serum levels of SF and LDH were detected by electrochemiluminescence immunoassay and rate method. 40 normal persons were selected as control group, and their serum SF and LDH levels were taken as controls. Results The incidence rate of bleeding symptoms in MA group was significantly lower than that in MDS group (15.00% vs 47.50%) (P< 0.05), but there was no significant difference in anemia or infection between these two groups (P> 0.05). The peripheral blood smears showed that the total visible cell count, proportions of normal and abnormal morphology of monocytes were significantly larger or higher, while the proportion of multiple segmented neutrophil was lower in MA group than MDS group (P< 0.05). There were significant differences between MA group than MDS group in granulocyte, erythroid and megakaryocyte dyshaematopoiesis (P< 0.05). The proportions of initial cells and karyotype abnormalities in MA group were significantly lower than those in MDS group (P< 0.05). Serum SF and LDH levels in MA group than MDS group were significantly higher than those in the control group (P< 0.05). The serum SF level was significantly lower, while the LDH level was significantly higher in MA group than MDS group (P< 0.05). Conclusion The serum SF level is significantly lower, while the LDH level is significantly higher in patients with MA than that in patients with MDS. There are similarities and differences in bone marrow cell morphology. Serum SF and LDH levels and bone marrow cell morphology are of significance for the differential diagnosis of MA and MDS.
  • The Influence and Correlation Analysis of Serum PLD, LXA4 and Gamma -GGT Levels in Type 2 Diabetic Peripheral Neuropathy
    LI Gai-xian 1, CHE Xiu-ying 2, ZHANG Hong 1, LIU Ping 1, HE Hui-ying 3, Ronia 4
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1658-1661. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.020
    Abstract ( ) Download PDF (2996KB) ( ) Knowledge map Save
    Objective To investigate the correlation between the levels of serum PLD, LXA4, and γ-GGT and type 2 diabetic peripheral neuropathy. Methods From January 2016 to June 2017, 60 patients with type 2 diabetes mellitus (T2DM) were selected as T2DM group. 60 patients with type 2 diabetic peripheral neuropathy were selected as DPN group and 50 healthy people as control group. The basic statisics and biochemical indexes of the three groups were compared, and the Pearson correlation and logistic multiple regression analyses were performed. Results The age, values of BMI, SBP, DBP, TC, TG, LDL-C, HDL-C, and FPG of the three groups were not significantly different (P>0.05). Compared with the control group, the course of disease, values of HbA1c and γ-GGT in group T2DM and DPN were significantly higher than those in the control group, and values of PLD and LXA4 were significantly lower than those in the control group (P<0.05). The course of disease, values of HbA1c and γ-GGT in group DPN were significantly higher than those in group T2DM, and values of PLD and LXA4 were significantly lower than those in group T2DM (P<0.05). After Pearson correlation analysis, the course of disease, values of HbA1c, and γ-GGT were positively correlated with the nerve conduction velocity of DPN, while PLD, LXA4 and DPN nerve conduction velocity were negatively correlated. The multiple regression analysis of logistic was carried out by stepwise regression, and the results showed that the course of disease, values of HbA1c, PLD, LXA4 and γ-GGT were the independent factors of DPN. Conclusion Serum PLD, LXA4 and γ -GGT are correlated with the occurrence of DPN, and γ-GGT may be its risk factor, and PLD, LXA4 may be the protective factors.
  • Relationship Between Vascular Endothelial Growth Factor, Macrophage Migration Inhibitory Factor and Atherosclerosis in Patients with Type 2 Diabetic Nephropathy
    NURONGGULI Maimaiti, AMANGULI Ezizi, WANG Yan, MA Yan-rong, GUO Yan-ying
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1662-1665. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.021
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    Objective To investigate the relationship between vascular endothelial growth factor, macrophage migration inhibitory factor and atherosclerosis in patients with type 2 diabetic nephropathy. Methods 150 patients with DN were selected from January 2016 to January 2018 as study group. A total of 100 healthy person were selected as control group. The basic statistics of the two groups were compared. The serum levels of VEGF, MIF in patients with different proteinuria and IMT were also compared, and logistic regression analysis was conducted. Results There was no significant difference in the sex ratio, age, BMI, and UA between the two groups. SBP, DBP, TC, TG, LDL-C, FPG, HbAlc, 24hUP, ITM, VEGF, and MIF in the study group were significantly higher than that in the control group, while HDL-C, eGFR in the study group were significantly lower than that in the control group (P<0.05). The level of serum VEGF, MIF in clinical proteinuria group and microalbuminuria group were significantly higher than that in control group (P<0.05). The levels of serum VEGF, MIF and ITM in the clinical proteinuria group were significantly higher than those in the microalbuminuria group (P<0.05). With the increase of IMT, the serum levels of VEGF and MIF were increased (P<0.05). Multivariate regression analysis showed that VEGF and MIF were  independent risk factors for DN complicated with AS. Conclusion The levels of serum VEGF and MIF are closely related to the degree of atherosclerosis in DN patients, which may be involved in the occurrence and development of atherosclerosis by promoting vascular inflammation.
  • The Clinical Curative Effect of Mirena Combined with Marvelon on Perimenopausal Dysfunctional Uterine Bleeding and their Influences on Serum Levels of FSH, LH, E2 and P
    YUAN Hua
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1666-1669. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.022
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    Objective To investigate the clinical curative effect of Mirena combined with Marvelon on perimenopausal dysfunctional uterine bleeding (DUB) and their influences on serum hormones levels. Methods 120 patients with perimenopausal DUB were randomly divided into the observation group and the control group, with 60 cases in each. The observation group were treated with Mirena combined with Marvelon while the control group were treated with Marvelon alone. The clinical curative effect, hemostasis, changes in serum hormones [follicle-stimulating hormone (FSH), luteinizing hormone (LH), estrogen (E2), progesterone (P)], endometrial thickness (ET) and hemoglobin (Hb) before and after treatment were compared between these two groups, and the medication safety was evaluated. Results The total treatment effective rate of the observation group was significantly higher than that of the control group (100.0% vs 90.0%) (P < 0.05). The time of vaginal bleeding control and the complete hemostasis time in the observation group were significantly shorter than those in the control group (P < 0.05). After treatment, serum levels of FSH, LH, E2 and P and ET in the observation group were significantly lower than those in the control group, while the level of Hb was significantly higher than that in the control group (P < 0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P > 0.05). Conclusion Mirena combined with Marvelon is effective in the treatment of perimenopausal DUB. The regimen can significantly improve levels of serum hormones, with high safety.
  • The Expressions of β-catenin and CD147 in Colorectal cancer and Their Correlations with Microvessel Density
    XIE Gang, WAGN An-qun, JI Yu-zhu, YANG Yong-hong
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1670-1673. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.023
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    Objective To investigate the expressions of β-catenin and CD147 in colorectal cancer and their correlations with the microvessel density. Methods From January 2017 to April 2018, 90 patients with primary colorectal cancer treated in our hospital were studied. At the same time, 90 patients with normal tissue adjacent to cancer were taken as the paracancerous group. The expressions of β-catenin and CD147 were  compared between the two groups and Spearman correlation analysis was performed. Results The abnormality rate of β-catenin(75.6%) and positive rate of CD147(83.3%) in colorectal cancer were  significantly higher than that in paracancerous group (12.2%, 23.3%). β-catenin, CD147 and MVD were not related with  sex, age, tumor diameter(P>0.05), which were related to TNM staging, degree of differentiation, distant metastasis, lymph node metastasis and serous infiltration (P<0.05). The MVD values of β-catenin (abnormal), CD147 (positive) and β-catenin(abnormal)+CD147 (positive) were significantly higher than those of β-catenin (normal), CD147 (negative) and β-catenin (normal) +CD147 (negative) (P<0.05), and the MVD value of beta -catenin (abnormal) +CD147 (positive) was the highest (P<0.05). By Pearson correlation analysis, the positive expression of β-catenin CD147 was positively correlated with MVD in colorectal carcinoma (P<0.05). Conclusion β-catenin and CD147 are related with  the occurrence and development of colorectal cancer, might  participate in tumor angiogenesis, invasion and metastasis.
  • The Diagnostic Values of miR-126, Transferrin, miR-133b, miR-342 and Cystatin C in Type 2 Diabetic Nephropathy
    ZHONG Hai-feng, ZHOU Yong-hua, HAN Xiao-jun, YANG Xiao-hua, SHEN Yi-mei
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1674-1678. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.024
    Abstract ( ) Download PDF (3837KB) ( ) Knowledge map Save
    Objective To investigate the diagnostic values of miR-126, Transferrin, miR-133b, miR-342 and cystatin C in type 2 diabetic nephropathy. Methods 30 patients with type 2 diabetic nephropathy were selected as the diabetic nephropathy group, while 30 patients with type 2 diabetes non-nephropathy were selected as the diabetic non-nephropathy group, and 30 healthy subjects were also selected as the control group. Three groups of serum miR-126 were then detected, as well as the  contents of Transferrin, miR-133b, miR-342 and cystatin C in urine. The area under the ROC curve was used to estimate  the diagnostic value of miR-126, Transferrin, miR-133b, miR-342 and cystatin C for type 2 diabetic nephropathy. Results The relative expression of miR-126 in diabetic nephropathy patients was significantly lower than that in diabetic non-nephrotic patients and healthy subjects (P<0.05), while the relative expression levels of miR-133b and miR-342 were correlated with cysstatin C and Transferrin. The expression level was significantly higher than that of patients with diabetes non-nephropathy and healthy subjects (P<0.05). The relative expression of miR-126 in diabetic non-nephropathy patients was significantly lower than that in healthy subjects (P<0.05), while the relative expressions of miR-133b, cystatin C and Transferrin were significantly higher than those in healthy subjects (P<0.05). The sensitivities of miR-126, miR-133b, miR-342, cystatin C and Transferrin in patients with type 2 diabetic nephropathy and non-type 2 diabetic nephropathy were 96%, 83%, 70%, 77%, and 83%, respectively; while the specificities  were 41%, 75%, 82%, 88%, and 80%, respectively. The sensitivity of the four markers joint tests was 97%, while the specificity was 77%, and the accuracy was 83%.Conclusion miR-126, Transferrin, miR-133b, miR-342 and cystatin C can be used as potential markers for the diagnosis of type 2 diabetic nephropathy.
  • A Study on the Relationship Between Expressions of CyclinD1, COX-2, and p53 and Helicobacter Pylori Infection in Gastric Cancer
    LI Man-yuan, ZHAO Jing-song
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1679-1682. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.025
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    Objective To investigate the relationship between expression levels of CyclinD1, COX-2, and p53 and helicobacter pylori infection in gastric cancer.  Methods A total of 102 gastric cancer patients in our hospital from January 2015 to June 2017 (the presence of Helicobacter pylori infection in the tissues) were selected as the infection group. We also included 98 patients with gastric cancer (there were no Helicobacter pylori infection in the tissues) as the non-infection group, and 100 cases of patients with normal gastric mucosa and excluding precancerous lesions as the control group. We then used Spearman correlation analysis and Logistic multivariate regression analysis to compare the factors and risk factors of helicobacter pylori infection in gastric cancer among groups. Results Compared to the control group and the non-infection group, the positive rate and expression of p53, CyclinD1 and COX-2 were higher in the infection group (P<0.05);  The positive rate and expression of P53, CyclinD1 and COX-2 were correlated to helicobacter pylori bacterial infection in gastric cancer tissues (P<0.05);  The above factors were independent risk factors for incidence of helicobacter pylori bacterial infection in gastric cancer (P<0.05). Conclusion CyclinD1, COX-2 and p53 are highly expressed in gastric cancer tissues infected with Helicobacter pylori. Therefore, detection of these indicators is useful for the early diagnosis and treatment of gastric cancer.
  • A Study of the Clinical Significance of Expression of Peripheral Blood HMGB-1 and NGAL in Prognosis of Patients with Septic Lung Cancer
    XU Zhi-hua 1,2, WANG Dong 1,2, JIA Chao 2
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1683-1687. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.026
    Abstract ( ) Download PDF (3862KB) ( ) Knowledge map Save
    Objective To investigate the clinical significance of the expression of HMGB-1 and NGAL in peripheral blood of patients with lung cancer with sepsis-induced lung damage.Methods A total of 196 patients with malignant tumors confirmed by the study were selected. Among them, 96 patients were concurrent lung injury with sepsis and the other 100 patients were non-septic lung cancer patients. By comparing the HMGB-1 and NGAL expression levels and prognosis of peripheral blood in two groups of patients, the clinical significance of the expression of HMGB-1 and NGAL in the prognosis of patients with septic lung cancer was analyzed. Data analysis was completed with SPSS18.0. Results In this study, 96 patients in the observation group and 100 patients in the control group were followed up. All the subjects were followed up for 2 years. 41 patients died in the observation group, with a mortality rate of 42.71%. In the control group, 30 patients were detected with a mortality rate of 30.00%.。Plasma levels of NGAL evaluation in patients with newly diagnosed results suggest that plasma NGAL levels in the observation group or patients died were significantly higher than that in the control group or patients survived, P <0.05. The levels of HMGB-1 in the newly diagnosed patients also showed that the level of serum HMGB-1 in the observation group was significantly higher than that in the control group (P <0.05), and the HMG-1 levels in the patients who were followed up less than 2 years because of death were higher than those of survivors, P <0.05. Further analysis, the sensitivity / specificity of serum HMGB-1 and NGAL in distinguishing pulmonary lesions from sepsis and non-sepsis were 78.3%/91.2% and 64.7%/76.1%, respectively, and the sensitivity of combined serum HMGB-1 and NGAL to sepsis and non-sepsis lung damage was 81.7%, and the specificity was 89.8%. The sensitivity / specificity of serum HMGB-1 and NGAL for the survival and death of patients after 2 years of identification were 80.1%/87.6% and 73.2%/62.4%, respectively, and the sensitivity of combined serum HMGB-1 and NGAL to the survival and death of patients after 2 years was 78.5% and the specificity was 90.4%. Conclusion There is a correlation between the high level of plasma NGAL and the presence of lung injury in sepsis and the positive association with poor prognosis. High levels of HMGB-1 are also closely associated with the presence of lung damage in sepsis and also positively associated with poor prognosis.
  • Short-term Curative Effect of Laparoscopic Radical Prostatectomy in the Treatment of Prostate Cancer and the Influence on Serum PSA, VEGF, IGF-I and FPSAR Levels
    XU Xiao-han, TANG Xian-fu
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1688-1691. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.027
    Abstract ( ) Download PDF (3769KB) ( ) Knowledge map Save
    Objective To explore the short-term curative effect of laparoscopic radical prostatectomy in the treatment of prostate cancer and the influence on serum prostate specific antigen (PSA), vascular endothelial growth factor (VEGF) and insulin-like growth factor I (IGF-I) levels. Methods 120 patients with prostate cancer were divided into experimental group and control group by random number table method, with 60 cases in each group. The two groups were treated by laparoscopic radical prostatectomy or open radical resection, respectively. Operative indexes (operative time, intraoperative blood loss, intestinal function recovery time, hospital stay), curative effect indexes [International Prostate Symptom Score (IPSS), the maximum flow rate, residual urine volume] and complications in the two groups were evaluated. Levels of serum total prostate specific antigen (t-PSA), free prostate specific antigen (f-PSA), VEGF and IGF-I were determined before operation and at 1 week after operation. The ratio of f-PSA/t-PSA (FPSAR) was calculated. Results  The operative time of the experimental group was significantly longer than that of the control group, while the intraoperative blood loss, intestinal function recovery time, hospital stay and the incidence of complications were significantly less/shorter/lower than those in the control group (P< 0.05). After operation, levels of t-PSA, f-PSA, VEGF and IGF-I in the two groups were decreased significantly while FPSAR increased significantly (P < 0.05). There was no significant difference in the IPSS score, the maximum urine flow rate, residual urine volume, t-PSA, f-PSA, VEGF, IGF-I or FPSAR at 1 week after operation (P> 0.05). Conclusion The laparoscopic radical prostatectomy can significantly downregulatelevels of serum PSA, VEGF and IGF-I in patients with prostate cancer and improve the FPSAR ratio. The short-term curative effect is similar to open radical operation. However, compared with open operation, the blood loss during laparoscopic operation is less, patients undergoing laparoscopic operation can recover more quickly, and complications are fewer and hospital stay is shorter.
  • The Estimated Value of Creatine Kinase-MB, Troponin T and Brain Natriuretic Peptide in Heart Injury for  Patients Treated with Chest Radiation-therapy
    GAO Dong-qi
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1692-1695. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.028
    Abstract ( ) Download PDF (3098KB) ( ) Knowledge map Save
    Objective to investigate the estimated value of creatine kinase-MB (CK-MB), troponin T (cTnT) and brain natriuretic peptide (BNP) in heart injury in patients treated with chest radiation-therapy. Methods 80 cases of patients underwent  chest radiation-therapy in our hospital were analyzed. All patients were treated with the threedimensional conformal radiation therapy. After the treatment, the radioactive heart injury,  levels of CK-MB. cTnT, BNP were estimated. Results Radioactive heart injury included tachycardia or bradycardia, T wave flat, ST segment depression, and I degree atrioventricular block. The rate of T wave flat was the highest  18.75%（15/80）. The II class of radioactive heart injury was the highest  15.00%（12/80）in our study. The levels of CK-MB, cTnT, and BNP 4 and 6 weeks after the radiotherapy were lower than before radiotherapy (P<0.05). Compared with the single detection, the joint detection had higher sensitivity, specificity, accuracy, positive predictive value, and negative predictive value (P<0.05). Conclusion The levels of CK-MB, cTnT, andBNP after the chest radiation-therapy are  lower.  The joint detection of CA199, CEA and AFP can increase the diagnostic efficiency in patients with ovarian cancer.
  • The Effect of Laparoscopic Radical Resection of Rectal Cancer on Levels of Serum MMP-9, TIMP-1 and Immune Cells in Patients with Rectal Cancer
    ZHU Zhi-ming
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1696-1699. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.029
    Abstract ( ) Download PDF (2969KB) ( ) Knowledge map Save
    Objective To explore the effect of laparoscopic radical resection of rectal cancer on serum matrix metalloproteinase -9 (MMP-9), tissue inhibitor of metalloproteinase-1 (TIMP-1) levels and cellular immune function in patients with rectal cancer. Methods 50 patients who underwent laparoscopic radical resection of rectal cancer and 50 patients received conventional open radical resection of rectal cancer in the hospital from March 2016 to March 2018 were selected as the study group and the control group, respectively. The operation associated indexes, serum MMP-9 and TIMP-1 and immune function indexes before and after the treatment were compared between the two groups. Results The intraoperative blood loss, postoperative exhaust time, length of hospital stay and the incidence of complications in the observation group were significantly less / shorter / lower than those in the control group (P< 0.05). There was no significant difference between the two groups for the operative time or the number of removed lymph nodes (P> 0.05). Serum MMP-9, CD4+ and CD4+/CD8+ in the two groups decreased significantly, while TIMP-1 and CD8+ increased significantly after the operation (P< 0.05). The levels of serum MMP-9 and CD8+ in the observation group were significantly lower than those in the control group, while TIMP-1, CD4+ and CD4+/CD8+ were significantly higher than those in the control group (P< 0.05). Conclusion Laparoscopic radical resection of rectal cancer can significantly reduce the level of MMP-9 and improve the level of TIMP-1, and it has  less influence on the immune function of patients.
  • To Study the Changes of Hemorheology, Coagulation, Anticoagulation and Fibrinolysis System in Patients with Cor Pulmonale at Acute Stage
    ZHANG Bang-you
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1700-1702. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.030
    Abstract ( ) Download PDF (2422KB) ( ) Knowledge map Save
    Objective To investigate the changes of hemorheology, coagulation, anticoagulation and fibrinolysis system in patients with cor pulmonale at acute stage. Methods From January 2015 to October 2016, 60 cases with chronic pulmonary heart disease were collected, including 30 cases of pulmonary heart disease in the remission stage and 30 cases of pulmonary heart disease in acute phase. 30 healthy cases were selected as control group. Blood coagulation, nticoagulation, fibrinolysis index and immune index were compared between two groups. Results ①Compared with the control group, the APTT in patients with cor pulmonale at remission stage was significantly lower, the D-D and FVIII were significantly higher, the data difference was obvious, and there was no significant difference (P>0.05). In the acute phase of cor pulmonale patients, PT and APTT levels were significantly lower, FVIII and D-D levels were significantly higher, and the data were significantly different, P<0.05. Compared with remission stage, PT and APTT in patients with acute stage were significantly lower, D-D and FVIII were significantly higher, and the difference of data was obvious, P<0.05. ②There was no significant difference in the levels of C3, C4, IgA, IgG and IgM between the two groups in the control group and the remission stage of pulmonary heart disease, P>0.05. Compared with the control group, levels of C3, C4, IgA, IgG and IgM in the acute phase of cor pulmonale were significantly lower, P<0.05. Conclusion The relationship between coagulation index,immune inflammatory factors and pulmonary heart disease disease closely, detective C3, C4, IgA, IgG, IgM, D-D level, blood coagulation index of patients with pulmonary heart disease, are conducive to the discovery and monitoring the progress of disease as soon as possible.
  • The Expression of Long Non-coding RNA MIAT in Breast Cancer with Different Molecular Subtypes and Its Relationships with Clinical Characteristics
    WU Ai-ran, WU Jing, LI Xiao-jie, ZHANG Zhao-xuan, WANG Yue, HE Jie
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1703-1706. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.031
    Abstract ( ) Download PDF (3648KB) ( ) Knowledge map Save
    Objective To investigate the expression of long non-coding RNA (lncRNA) MIAT in different molecular subtypes of breast cancer, and to analyze the relationships between its expression and the clinical features of breast cancer. Methods The breast cancer tissue, para cancer tissue and peripheral blood of 212 patients with breast cancer diagnosed in our hospital from May 2015 to September 2017 were collected and the expressions of lncRNA MIAT in tissue and plasma were detected by quantitative real-time quantitative PCR (RT-PCR). The expression levels of lncRNA MIAT in cancer and paracancerous tissues were compared, and the relationships between its expression and molecular types, TNM stages, tumor size, number of axillary lymph nodes and other clinicopathological characteristics were analyzed. Results The expression level of lncRNA MIAT in breast cancer tissues (4.23±1.06) was significantly higher than that of para cancerous tissue (2.14±0.63)(P<0.05). In addition, the expression levels of lncRNA MIAT in cancer tissues and plasma were significantly correlated with the tumor molecular classification, TNM stages, tumor diameter, and the number of axillary lymph node metastases (P<0.05), but not related to the patient's age, BMI, smoking history, drinking history, birth history, breast feeding history (P>0.05). Conclusion The expression level of lncRNA MIAT was significantly related to the molecular classification of breast cancer, TNM stage, tumor diameter and the number of axillary lymph node metastases, which are of great value for the prognosis of patients.
  • A Study of the Changes of Serum Erythropoietin Level and Ferritin Expression in Elderly Patients with Leukemia and Its Clinical Significance
    LIU Lian
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1707-1711. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.032
    Abstract ( ) Download PDF (4458KB) ( ) Knowledge map Save
    Objective To investigate the changes of serum erythropoietin and ferritin expression in elderly patients with leukemia and to provide a reference for the development of clinical diagnostic criteria of elderly patients with leukemia and the subsequent personalized treatment plans. Methods From July 2015 to December 2016, cases of leukemia were collected from Department of Hematology and Oncology of a Grade III Hospitals for patients of ≥60 years old by means of case sampling. Combined with cross-sectional investigation and randomized controlled clinical trial (The treatment regimen included AML, M3 and stem cell transplantation, and the placebo-controlled design in the control group) to evaluate the diagnostic value of serum erythropoietin and ferritin levels in patients with senile leukemia, so to assess the changes of these two indicators before and after the intervention and its clinical significance. Results The effective sample size obtained in this study was 208 cases. The levels of serum erythropoietin and ferritin in elderly patients with leukemia were higher than those in normal controls. The results of paired χ2  test showed no differences between the 2 screening indexes of serum erythropoietin and the expression of ferritin as the diagnostic criteria of leukemia MICH (P>0.05). The 3 treatments used in this study could effetely reduce the level of serum erythropoietin and the level of ferritin expression in elderly patients with leukemia (All P <0.05). Conclusions The serum erythropoietin (EPO) level and the increase of ferritin expression level in the elderly patients with leukemia are related to the elderly leukemia. Therefore, both indexes have promising diagnostic and screening values, and the combined use of two for the initial diagnosis of elderly leukemia can be considered. Meanwhile, all the major treatment regimens are effective for improving serum erythropoietin level and ferritin level in elderly patients with leukemia with different performances. Based on the patient's condition, a suitable treatment plan can be developed.
  • Clinical Efficacy of Bailing Capsule Combined with GP Method on Elderly Patients with Non Small Cell Lung Cancer and its influence on the levels of CA125, CEA and VEGF in patients
    CHEN Xu
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1712-1715. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.033
    Abstract ( ) Download PDF (3650KB) ( ) Knowledge map Save
    Objective To study the clinical efficacy of bailing capsule combined with GP method on elderly patients with non small cell lung cancer (NSCLC) and its influence on the levels of CA125, CEA and VEGF in patients. Methods 76 elderly patients with NSCLC from our hospital were randomly divided into observation group and control group, with 38 cases in each group. Control group was treated with GP method, and observation group was given bailing capsule on the base of control group. After the treatment, the objective response rate and clinical benefit rate of two groups were observed. Before and after treatment, the levels of immune index (IgM, IgG and IgA), CA125, CEA and VEGF in the 2 groups were detected. During the treatment, the rates of adverse reactions in the 2 groups were observed, including rashes, leukopenia, gastrointestinal reactions, thrombocytopenia and neurotoxicity. Results The objective response rate and clinical benefit rate of observation group were 92.1% and 86.8%, and significantly higher than those in control group (P<0.05). After the treatment, the levels of IgM, IgG and IgA were significantly higher in observation group than those in control group (P<0.05), while the levels of CA125, CEA and VEGF were significantly lower in observation group than those in control group (P<0.05). During the treatment, the rates of rashes, leukopenia, thrombocytopenia and neurotoxicity in observation group were significantly lower in observation group than those in control group (P<0.05). Conclusions The method of bailing capsule combined with GP method on elderly patients with NSCLC can improve the clinical efficacy, and be worthy of following study.
  • A Study of CYFRA21-1,β2-MG and SCC-Ag Expression Levels and Their  Relationships with Efficacy of Chemotherapy and Prognosis in Patients with Advanced Non-small Cell Lung Cancer
    LU You-guo, YE Bing, XIA Li-li
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1716-1720. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.034
    Abstract ( ) Download PDF (3677KB) ( ) Knowledge map Save
    Objective To investigate the potential correlations between cytokeratin 19 (CYFRA21-1), β2-microglobulin (β2-MG) and squamous cell carcinoma antigen (SCC-Ag) levels and the efficacy and prognosis of non-small cell lung cancer (NSCLC) chemotherapy. Methods 60 patients with advanced non-small cell lung cancer treated in our hospital from January 2015 to December 2017 were selected as the study subjects. All patients were treated with first-line chemotherapy for non-small cell lung cancer. The serum levels of CYFRA21-1, β2-MG and SCC-Ag were compared before and after chemotherapy,  as well as the related prognosis. Results Compared with before chemotherapy, the serum levels of CYFRA21-1, β2-MG and SCC-Ag in patients with adenocarcinoma and squamous cell carcinoma were significantly lower after chemotherapy (P<0.05). After chemotherapy, there were 12 cases of complete remission (CR), 17 cases of partial remission (PR) 13 cases of stable disease (SD) and 18 cases of Disease progression (PD). The levels of CYFRA21-1, β2-MG, and SCC-Ag in patients with CR , PR and SD were significantly lower (P<0.05), while the levels of CYFRA21-1, β2-MG and SCC-Ag in PD increased significantly (P<0.05). The mean progression-free survival (PFS) and overall survival (OS) of patients with CYFRA21-1 ≥5 mg/L were significantly shorter than those with CYFRA21-1 <5 mg/L, (P<0.05). The mean PFS and OS of β2-MG≥4mg/L were significantly shorter than those of β2-MG<4mg/L (P<0.05); For the patients with SCC-Ag≥2mg/L, the average of PFS and OS were significantly shorter than patients with SCC-Ag <2 mg/L, (P<0.05). The median survival time of the CR group was 571 days, and the 1, 2 and 3-year survival rates were 61.3%, 34.7% and 18.9%, respectively. The median survival time of the PR, SD and PD group were 422 days, 339 days, and 303 days, respectively. The 3-year survival rate was 10.1%, 0%, and 0%, respectively. Conclusion CYFRA21-1, β2-MG and SCC-Ag levels can be used as the clinical predictors of chemotherapy efficacy and prognosis in NSCLC patients.
  • Effect of Trimebutine Maleate Tablets Combined with Compound Eosinophil-Lactobacillus Tablets on Gastrointestinal Hormones in Patients with Irritable Bowel Syndrome
    GAO Wei-na1，LIU Chao1，YUAN Qin2
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1721-1724. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.035
    Abstract ( ) Download PDF (3664KB) ( ) Knowledge map Save
    Objective  To explore the effect of trimebutine maleate tablets combined with compound eosinophil-lactobacillus tablets on gastrointestinal hormones in patients with irritable bowel syndrome (IBS). Methods 120 patients with diarrhea-predominant IBS (IBS-D) who were admitted to the hospital from March 2015 to March 2017 were randomly divided into observation group and control group by the random number table method, with 60 cases in each group. Control group were treated with trimebutine maleate tablets, while observation group were treated with trimebutine maleate tablets combined with compound eosinophil-lactobacillus tablets. The two groups were given 30d of treatment. The clinical curative effect, changes of symptom scores, gastrointestinal hormones and inflammatory cytokines before and after the treatment were compared between the two groups. Results The effective rate of treatment in the observation group was higher than that in the control group (93.33% vs 80.0%) (P < 0.05). The scores of diarrhea and abdominal pain in the observation group after treatment were lower than those in the control group (P < 0.05). After treatment, levels of plasma 5--hydroxytryptamine (5-HT), plasma neuropeptide Y (NPY) and somatostatin (SS) in the observation group were lower than those in the control group, while the plasma motilin (MOT) level was significantly higher than that in the control group (P < 0.05). After treatment, the serum level of tumor necrosis factor α (TNF- α) in the observation group was lower than that in the control group, while the level of interleukin 10 (IL-10) was higher than that in the control group (P < 0.05). Conclusion The combined treatment of trimebutine maleate tablets and compound eosinophil-lactobacillus tablets for IBS-D can significantly relieve symptoms such as abdominal pain and diarrhea, and the effect is definite, which may be related to adjusting levels of gastrointestinal related hormones and reducing levels of inflammatory cytokines.
  • Effect of Fluvastatin Combined with Valsartan on the Microinflammatory State of Patients with Peritoneal Dialysis
    CAI Hong-li1, ZHANG Rui-ying1, DUAN Yu-jia2, YANG Li-juan1, CHEN Qiu-yue1
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1725-1729. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.036
    Abstract ( ) Download PDF (4195KB) ( ) Knowledge map Save
    Objective To study the effect of fluvastatin combined with valsartan on the microinflammatory state of patients with peritoneal dialysis. Methods 150 patients with peritoneal dialysis were randomly divided into three groups, with 50 patients in each group. The control group was given conventional peritoneal dialysis. The valsartan group was treated with oral 80mg/d valsartan tablets on the basis of conventional peritoneal dialysis treatment. On the basis of routine peritoneal dialysis treatment, the fluvastatin combined with valsartan group was given oral 80 mg valsartan and 20 mg fluvastatin once daily for 24 weeks. To detect the inflammatory response indicators, blood lipids and nutritional status indicators were analysed before and after treatment of the three groups. Results C-reactive protein (CRP), interleukin-6 (IL-6) , interleukin-8 (IL-8), tumor necrosis factor-α (TNF-α), serum amyloid A (SAA), pregnancy associated plasma protein-A (PAPP-A) and cholinesterase (CHE) levels in the valsartan group and fluvastatin combined with valsartan group after treatment were significantly lower than those before treatment. Serum levels of CRP、IL-6、IL-8、TNF-α、SAA、PAPP-A and CHE in the fluvastatin combined with valsartan group after treatment were significantly lower than those in valsartan group; The triglyceride (TG), total cholesterol (TC), and low-density lipoprotein (LDL) levels in the valsartan group and fluvastatin combined with valsartan group after treatment were significantly lower than those before treatment, and the level of high density lipoprotein (HDL) increased significantly. The TG、TC and LDL levels in the fluvastatin combined with valsartan group was significantly lower than those in the control group and valsartan group, and the HDL levels were significantly higher. After treatment, serum albumin (Alb), prealbumin (PA), and tissue factor (TF) levels in the fluvastatin combined with valsartan group were not significantly different from those before treatment, while serum Alb, PA and Tf levels in control group and valsartan group after treatment were significantly lower than those before treatment. Conclusion Fluvastatin combined with valsartan has good effect on the treatment of micro-inflammation in peritoneal dialysis patients, and has a certain clinical application value.
  • The Characteristics of Peripheral Blood γδ T Cell Proportion and Its Apoptosis in Children with Neuroblastoma
    WANG Xiao-lin,MOU Wen-jun,CHEN Xi,ZHANG Hui,GUI Jin-gang
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1730-1735. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.037
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    Objective The aim of this study is to investigate the proportion of γδ T cells and concentrations of multiple cytokines in peripheral blood of neuroblastoma patients. Furthermore,  we will also stimulate γδ T cell proliferation and analyze the apoptosis of γδ T cells. Methods The proportion of peripheral blood γδ T cells (CD3+、TCRγδ+) from children with NB was  analyzed through flow cytometry. The concentrations of multiple cytokines were detected by luminex200 system. We then stimulated and cultured γδ T cells in the condition of PAM and IL-2, and detected the cell apoptosis by Annexin V staining. Results The γδ T cells proportion of NB patients were up-regulated significantly and cytokines with survival promoting effect in serum were down-regulated. Moreover, we found an increase of the apoptosis of NB patient γδ T cells during in vitro culture. Conclusion Our research revealed a pathological augment of γδ T cell proportion and its apoptosis in NB patients, which might due to the decrease of cytokines with survival promoting effect. With that knowledge, we proposed that enhancing  the anti-apoptosis ability of γδ T cell is significant to its application to NB clinic treatment.
  • A Study of The Clinical Diagnostic Value Three Kinds of Methodologies for Syphilis Patients Serum and Cerebrospinal Fluid Evaluation
    XU Fei,LIU Ying, ZHOU Chun, Wang Ya-Jie
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1736-1739. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.038
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    Objective To study the syphilis patients serum and cerebrospinal fluid for the sensitivity/specificity and correlations in detection methodology, so to evaluate three kinds of methodologies for syphilis especially the diagnostic value of neurosyphilis Method In 483 cases, TRUST (toluidine red unheated test), TPPA (syphilis gelatin particle agglutination test), and FTA - ABS treponema pallidum (fluorescence detection) used to test syphilis serum and cerebrospinal fluid. Patients with syphilis serology positive were given the regular flooding treatment. After six months, we reviewed and evaluated three methodologies separately and the joint detection for the sensitivity and specificity. Result Among 483 patients, there were 372 cases of patients with positive serum for syphilis. For TRUST to detect syphilis serum, the sensitivity and specificity were 68.64% and 88.34% respectively, TPPA syphilis detection of 100% and 99%., and  FTA - ABS detection of 100%. For serum levels of joint detection, the sensitivity and specificity were 100%. There were 52 patients with cerebrospinal fluid positive diagnosis of neurosyphilis. For neurosyphilis cerebrospinal fluid,  TRUST had the sensitivity and specificity of 88.64% and 98.13% respectively, TPPA of 100% and 99% respectively, and FTA - ABS of 96.4% and 100% respectively. The cerebrospinal fluid joint detection had the sensitivity and specificity of 100%. After six months, in 52 cases of cerebrospinal fluid, TRUST TPPA and FTA ABS were used for reviewing. TRUST overcast rate was 40.38% (21/52), and degree of decline rate was 71.15% (37/52), and drops of < 1:4, TPPA and FTA - ABS were positive, and serum TRUST overcast rate was 7.69% (4/52), drops fell rate was 51.92% (27/52), drop degree < 1:16. Cerebrospinal fluid TRUST overcast rate was relatively higher than blood TRUST turns shade rate, (P < 0.05). Conclusion CSF TRUST can be used as for curative effect observation, also the index of relapse or reinfection. Fluorescence treponema pallidum test methodology, as the confirmed experiment, has certain advantages, but the positive rate is slightly lower than the TPPA in cerebrospinal fluid examination. It should be jointly used with TPPA as neurosyphilis standard of for confirming cases. The laboratory cerebrospinal fluid syphilis screening diagnosis should give the priority to TPPA, joint TRUST as a clinical observation, and joint FTA - ABS as syphilis diagnosis experiment.
  • The Verification of Analytical  Performance of EASYCUTA by the Time-resolved Fluorecence Immunoassay
    HUANG Rui, JIA Hai-ying, WANG Chang-min
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1740-1746. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.039
    Abstract ( ) Download PDF (5198KB) ( ) Knowledge map Save
    Objective The aim of study is to establish the program and methods for the performance verification of time-resolved fluoroimmuno assay(TRFIA). Methods According to the relevant?requirements of Accreditation criteria for quality and capacity in medical laboratories(ISO 15189:2012), combined with the actual work requirements, we designed the experimental verification program. Hepatitis B virus markers(HBV-M), Syphilis antibody(TP-Ab), Hepatitis C virus antibody(HCV-Ab) and Human immunodeficiency virus antibody(HIV-Ab) were detected by EASYCUTA analyzer. The precision, accuracy, measurement range, clinical reportable range, biological reference intervals, sensitivity, interference immunity, cross-contamination and methodology comparison were obtained and analyzed. The test results were compared with those provided by the manufacturer (Suzhou XinBo Corporation). Results For  the HBV-M, TP-Ab, HCV-Ab, HIV-Ab test results, the precision, accuracy, measurement range, clinical reportable range, biological reference intervals, sensitivity, interference immunity, cross-contamination of EASYCUTA were all consistent with the international standards . For the methodological comparison, the coincidence rate of HBV test with Roche e601 was greater than 95%, and the K value was rated as excellent. The?four?markers?of?the?infectious?diseases (HBsAg, TP, HCV, HIV)with Abbott i2000 was greater than 95%, and the K value was rated as excellent. So the methodological comparisons were qualified. Conclusion The performance verification results that include HBV-M, TP-Ab, HCV-Ab, and HIV-Ab of EASYCUTA were consistent with the analytical performance defined by the manufacturer. It can meet the clinical requirements. This study provides very useful knowledge base for standardizing the construction of medical laboratory and estimating the quality of instrument testing.
  • The Analysis and Nursing Strategy of Depression and Anxiety in Thyroid Cancer Patients After Radioiodine 131 Treatment
    GUO Qing-ling, HAN Xiao-xia, ZHANG Xiao-na, ZHAO ling, ZHI Jun-jie
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1747-1749. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.040
    Abstract ( ) Download PDF (2545KB) ( ) Knowledge map Save
    Objective  To investigate the incidence of depression and anxiety in thyroid cancer patients after the radioiodine 131 treatment and provide reference data for future nursing methods and research. Methods Using Zung self-rating depression scale and Zung self-rating anxiety scale, we examined the incidence of depression and anxiety in 160 post operation thyroid cancer patients, hospitalized for the radioiodine 131 internal radiation therapy from January 2015 to July 2017. Results The incidence of depression in thyroid cancer patients was up to 77% and 47% of them were severe depression. The incidence of anxiety in thyroid cancer patients was relatively lower than the  incidence of depression. Conclusion The incidence of depression in thyroid cancer patients duringthe radioiodine 131 treatment is very high. In addition to the routine treatment and nursing care, it is more important to carry out psychological care for them. Satisfactory results can be achieved by allowing patients to develop confidence during treatments and build up the courage for their lives.
  • The Progress in Clinical Research of 177Lu Labeled Radiopharmaceuticals
    FENG Ting-ting1,CHENG Wei-hua1,WANG Bin2,FAN Hong-qiang1
    Labeled Immunoassays and Clinical Medicine. 2018, 25(11): 1750-1756. https://doi.org/10.11748/bjmy.issn.1006-1703.2018.11.041
    Abstract ( ) Download PDF (4868KB) ( ) Knowledge map Save
    177Lu has great advantages in the field of tumor therapy because of its excellent radioactive physical properties. A large number of clinical studies have been carried out by clinical researchers on 177Lu labeled radiopharmaceuticals. 177Lu-labeled somatostatin analogue 177Lu-DOTATATE has been approved both in the United States and the European Union. This article reviews the clinical research progress of six 177Lu-labeled radiopharmaceuticals including their labeled peptides, monoclonal antibodies and compounds. Some of them have been approved for clinical trials and others have not been endorsed. Clinical data showed that some of the drugs listed in this article have relatively good safety, and have promising effects on prolonging the survival of patients and improving patient’s quality of life. Some drug usage has been proved to be safe, but its specific clinical efficacy needs to be supported by more clinical trial data. We are expecting more 177Lu labeled drugs to enter the market in the near future.
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Monthly, Established in February 1994
Responsible Institution
China National Nuclear Corporation
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China Isotope & Radiation Corporation
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Editorial Board of Labeled Immunoassays and Clinical Medicine
Editor-in-Chief: TIAN Yaping
ISSN 1006-1703
CN 11-3294/R
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